Interaction between the human nuclear cap-binding protein complex and hnRNP F.
hnRNP F was identified in a screen for proteins that interact with human CBP80 and CBP20, the components of the nuclear cap-binding complex (CBC). In vitro interaction studies showed that hnRNP F can bind to both CBP20 and CBP80 individually. hnRNP F and CBC bind independently to RNA, but hnRNP ... F binds preferentially to CBC-RNA complexes rather than to naked RNA. The hnRNP H protein, which is 78% identical to hnRNP F and also interacts with both CBP80 and CBP20 in vitro, does not discriminate between naked RNA and CBC-RNA complexes, showing that this effect is specific. Depletion of hnRNP F from HeLa cell nuclear extract decreases the efficiency of pre-mRNA splicing, a defect which can be partially compensated by addition of recombinant hnRNP F. Thus, hnRNP F is required for efficient pre-mRNA splicing in vitro and may participate in the effect of CBC on pre-mRNA splicing.
Mesh Terms:
Amino Acid Sequence, Cross-Linking Reagents, Electrophoresis, Polyacrylamide Gel, Hela Cells, Heterogeneous-Nuclear Ribonucleoprotein Group F-H, Heterogeneous-Nuclear Ribonucleoproteins, Humans, Macromolecular Substances, Molecular Sequence Data, RNA Cap-Binding Proteins, RNA Caps, RNA, Heterogeneous Nuclear, RNA-Binding Proteins, Ribonucleoproteins
Amino Acid Sequence, Cross-Linking Reagents, Electrophoresis, Polyacrylamide Gel, Hela Cells, Heterogeneous-Nuclear Ribonucleoprotein Group F-H, Heterogeneous-Nuclear Ribonucleoproteins, Humans, Macromolecular Substances, Molecular Sequence Data, RNA Cap-Binding Proteins, RNA Caps, RNA, Heterogeneous Nuclear, RNA-Binding Proteins, Ribonucleoproteins
Mol. Cell. Biol.
Date: May. 01, 1997
PubMed ID: 9111328
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