A newly identified essential complex, Dre2-Tah18, controls mitochondria integrity and cell death after oxidative stress in yeast.
A mutated allele of the essential gene TAH18 was previously identified in our laboratory in a genetic screen for new proteins interacting with the DNA polymerase delta in yeast [1]. The present work shows that Tah18 plays a role in response to oxidative stress. After exposure to lethal doses of ... H(2)O(2), GFP-Tah18 relocalizes to the mitochondria and controls mitochondria integrity and cell death. Dre2, an essential Fe/S cluster protein and homologue of human anti-apoptotic Ciapin1, was identified as a molecular partner of Tah18 in the absence of stress. Moreover, Ciapin1 is able to replace yeast Dre2 in vivo and physically interacts with Tah18. Our results are in favour of an oxidative stress-induced cell death in yeast that involves mitochondria and is controlled by the newly identified Dre2-Tah18 complex.
Mesh Terms:
Amino Acid Sequence, Gene Deletion, Gene Dosage, Genes, Suppressor, Green Fluorescent Proteins, Humans, Hydrogen Peroxide, Intracellular Signaling Peptides and Proteins, Iron-Sulfur Proteins, Microbial Viability, Mitochondria, Molecular Sequence Data, Mutagens, Mutant Proteins, Oxidative Stress, Protein Binding, Protein Transport, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature
Amino Acid Sequence, Gene Deletion, Gene Dosage, Genes, Suppressor, Green Fluorescent Proteins, Humans, Hydrogen Peroxide, Intracellular Signaling Peptides and Proteins, Iron-Sulfur Proteins, Microbial Viability, Mitochondria, Molecular Sequence Data, Mutagens, Mutant Proteins, Oxidative Stress, Protein Binding, Protein Transport, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature
PLoS ONE
Date: Feb. 06, 2009
PubMed ID: 19194512
View in: Pubmed Google Scholar
Download Curated Data For This Publication
94825
Switch View:
- Interactions 6