Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.

Cadherins are Ca(2+)-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, transmembrane cadherins such as E-, P-, and N-cadherin self-associate, while intracellularly they interact indirectly with the actin-based cytoskeleton. Several intracellular proteins termed catenins, including alpha-catenin, beta-catenin, and plakoglobin, are tightly associated with these cadherins and serve to link them ...
to the cytoskeleton. Here, we present evidence that in fibroblasts alpha-actinin, but not vinculin, colocalizes extensively with the N-cadherin/catenin complex. This is in contrast to epithelial cells where both cytoskeletal proteins colocalize extensively with E-cadherin and catenins. We further show that alpha-actinin, but not vinculin, coimmunoprecipitates specifically with alpha- and beta-catenin from N- and E-cadherin-expressing cells, but only if alpha-catenin is present. Moreover, we show that alpha-actinin coimmunoprecipitates with the N-cadherin/catenin complex in an actin-independent manner. We therefore propose that cadherin/catenin complexes are linked to the actin cytoskeleton via a direct association between alpha-actinin and alpha-catenin.
Mesh Terms:
Actinin, Cadherins, Calcium, Cell Adhesion, Cell Line, Cytoskeletal Proteins, Cytoskeleton, Fibroblasts, Fluorescent Antibody Technique, Humans, Macromolecular Substances, Precipitin Tests, Vinculin, alpha Catenin
J. Cell Biol.
Date: Jul. 01, 1995
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