Conserved telomere maintenance component 1 interacts with STN1 and maintains chromosome ends in higher eukaryotes.
Orthologs of the yeast telomere protein Stn1 are present in plants, but other components of the Cdc13/Stn1/Ten1 (CST) complex have only been found in fungi. Here we report the identification of conserved telomere maintenance component 1 (CTC1) in plants and vertebrates. CTC1 encodes an approximately 140 kDa telomere-associated protein predicted ... to contain multiple OB-fold domains. Arabidopsis mutants null for CTC1 display a severe telomere deprotection phenotype accompanied by a rapid onset of developmental defects and sterility. Telomeric and subtelomeric tracts are dramatically eroded, and chromosome ends exhibit increased G overhangs, recombination, and end-to-end fusions. AtCTC1 both physically and genetically interacts with AtSTN1. Depletion of human CTC1 by RNAi triggers a DNA damage response, chromatin bridges, increased G overhangs, and sporadic telomere loss. These data indicate that CTC1 participates in telomere maintenance in diverse species and that a CST-like complex is required for telomere integrity in multicellular organisms.
Mesh Terms:
Anaphase, Arabidopsis, Arabidopsis Proteins, Cell Line, Tumor, Chromosomal Proteins, Non-Histone, Chromosomes, Plant, Conserved Sequence, Eukaryotic Cells, Genomic Instability, Humans, In Situ Hybridization, Fluorescence, Mutation, Nucleic Acid Conformation, Protein Binding, Recombination, Genetic, Telomere, Telomere-Binding Proteins
Anaphase, Arabidopsis, Arabidopsis Proteins, Cell Line, Tumor, Chromosomal Proteins, Non-Histone, Chromosomes, Plant, Conserved Sequence, Eukaryotic Cells, Genomic Instability, Humans, In Situ Hybridization, Fluorescence, Mutation, Nucleic Acid Conformation, Protein Binding, Recombination, Genetic, Telomere, Telomere-Binding Proteins
Mol. Cell
Date: Oct. 23, 2009
PubMed ID: 19854131
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