A general life-death selection strategy for dissecting protein functions.

Clonal selection strategies are central tools in molecular biology. We developed a general strategy to dissect protein functions through positive and negative clonal selection for protein-protein interactions, based on a protein-fragment complementation assay using Saccharomyces cerevisiae cytosine deaminase as a reporter. We applied this method to mutational or chemical disruption ...
of protein-protein interactions in yeast and to dissection of the functions of an allosterically activated transcription factor, Swi6.
Mesh Terms:
Cytosine Deaminase, Genetic Complementation Test, Peptide Fragments, Protein Interaction Mapping, Saccharomyces cerevisiae
Nat. Methods
Date: Nov. 01, 2009
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