P66(ShcA) interacts with MAPKAP kinase 2 and regulates its activity.

Department of Inflammation, Wyeth Research, 200 Cambridge Park Drive, Cambridge, MA 02140-2311, USA. yvonne.yannoni@abbott.com
Three mitogen activated protein kinase-activated protein kinase 2 (MAPKAP kinase 2, MK2) interacting proteins were identified using a yeast two-hybrid approach. ShcA, a signaling phospho-protein, human polyhomeotic 2 (HPH2), a transcriptional regulator, and highly similar to smoothelin (HSTS), which is related to the cytoskeletal associated protein smoothelin, interact specifically with MK2. The interaction of MK2 with the 66 kDa isoform of ShcA, p66(ShcA), and HPH2 was confirmed using co-immunoprecipitation. MK2 is activated with p66(ShcA) co-expression and p66(ShcA) is an in vitro substrate for MK2, further demonstrating their association and suggesting a biological role for p66(Shc) in MK2 activation.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Humans, Intracellular Signaling Peptides and Proteins, Molecular Sequence Data, Nuclear Proteins, Phosphoproteins, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases, Sequence Alignment, Shc Signaling Adaptor Proteins, Transcription Factors, Two-Hybrid System Techniques
FEBS Lett. Apr. 23, 2004; 564(1);205-11 [PUBMED:15094067]
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