Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription.
Human PC4 and the yeast ortholog Sub1 have multiple functions in RNA polymerase II transcription. Genome-wide mapping revealed that Sub1 is present on Pol III-transcribed genes. Sub1 was found to interact with components of the Pol III transcription system and to stimulate the initiation and reinitiation steps in a system ... reconstituted with all recombinant factors. Sub1 was required for optimal Pol III gene transcription in exponentially growing cells.
Mesh Terms:
Blotting, Far-Western, Chromatin Immunoprecipitation, Chromosome Mapping, Chromosomes, Fungal, DNA, Fungal, DNA-Binding Proteins, Gene Expression Regulation, Developmental, Gene Expression Regulation, Fungal, Genome, Fungal, Mutation, Oligonucleotide Array Sequence Analysis, Protein Binding, RNA Polymerase III, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription Factors, TFIII, Transcription, Genetic
Blotting, Far-Western, Chromatin Immunoprecipitation, Chromosome Mapping, Chromosomes, Fungal, DNA, Fungal, DNA-Binding Proteins, Gene Expression Regulation, Developmental, Gene Expression Regulation, Fungal, Genome, Fungal, Mutation, Oligonucleotide Array Sequence Analysis, Protein Binding, RNA Polymerase III, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription Factors, TFIII, Transcription, Genetic
Proc. Natl. Acad. Sci. U.S.A.
Date: Aug. 25, 2009
PubMed ID: 19706510
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