Potentiation of serum response factor activity by a family of myocardin-related transcription factors.
Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac ... and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Blotting, Northern, Cloning, Molecular, DNA, Complementary, Expressed Sequence Tags, Gene Expression Regulation, Developmental, Glutathione Transferase, Humans, Mice, Molecular Sequence Data, Myocardium, Nuclear Proteins, Recombinant Fusion Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Serum Response Factor, Trans-Activators, Transcription Factors, Xenopus
Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Blotting, Northern, Cloning, Molecular, DNA, Complementary, Expressed Sequence Tags, Gene Expression Regulation, Developmental, Glutathione Transferase, Humans, Mice, Molecular Sequence Data, Myocardium, Nuclear Proteins, Recombinant Fusion Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Serum Response Factor, Trans-Activators, Transcription Factors, Xenopus
Proc. Natl. Acad. Sci. U.S.A.
Date: Nov. 12, 2002
PubMed ID: 12397177
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