Interaction of ATF6 and serum response factor.

Serum response factor (SRF) is a transcription factor which binds to the serum response element (SRE) in the c-fos promoter. It is required for regulated expression of the c-fos gene as well as other immediate-early genes and some tissue-specific genes. To better understand the regulation of SRF, we used a ...
yeast interaction assay to screen a human HeLa cell cDNA library for SRF-interacting proteins. ATF6, a basic-leucine zipper protein, was isolated by binding to SRF and in particular to its transcriptional activation domain. The binding of ATF6 to SRF was also detected in vitro. An ATF6-VP16 chimera activated expression of an SRE reporter gene in HeLa cells, suggesting that ATF6 can interact with endogenous SRF. More strikingly, an antisense ATF6 construct reduced serum induction of a c-fos reporter gene, suggesting that ATF6 is involved in activation of transcription by SRF. ATF6 was previously partially cloned as a member of the ATF family. The complete cDNA of ATF6 was isolated, and its expression pattern was described.
Mesh Terms:
Activating Transcription Factor 6, Amino Acid Sequence, Animals, Binding Sites, COS Cells, Cloning, Molecular, DNA Footprinting, DNA-Binding Proteins, Genes, Reporter, Genes, fos, Hela Cells, Humans, Molecular Sequence Data, Nuclear Proteins, Oligonucleotides, Antisense, Promoter Regions, Genetic, Protein Binding, Recombinant Proteins, Saccharomyces cerevisiae, Sequence Analysis, DNA, Serum Response Factor, Transcription Factors, Transcriptional Activation
Mol. Cell. Biol.
Date: Sep. 01, 1997
Download Curated Data For This Publication
9583
Switch View:
  • Interactions 4