A mechanism for chromosome segregation sensing by the NoCut checkpoint.
In Saccharomyces cerevisiae and HeLa cells, the NoCut checkpoint, which involves the chromosome passenger kinase Aurora B, delays the completion of cytokinesis in response to anaphase defects. However, how NoCut monitors anaphase progression has not been clear. Here, we show that retention of chromatin in the plane of cleavage is ... sufficient to trigger NoCut, provided that Aurora/Ipl1 localizes properly to the spindle midzone, and that the ADA histone acetyltransferase complex is intact. Furthermore, forcing Aurora onto chromatin was sufficient to activate NoCut independently of anaphase defects. These findings provide the first evidence that NoCut is triggered by the interaction of acetylated chromatin with the passenger complex at the spindle midzone.
Mesh Terms:
Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Cytokinesis, Endopeptidases, Histone Acetyltransferases, Intracellular Signaling Peptides and Proteins, Microtubules, Mitotic Spindle Apparatus, Protein Binding, Protein Kinases, Protein Transport, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Cytokinesis, Endopeptidases, Histone Acetyltransferases, Intracellular Signaling Peptides and Proteins, Microtubules, Mitotic Spindle Apparatus, Protein Binding, Protein Kinases, Protein Transport, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Nat. Cell Biol.
Date: Apr. 01, 2009
PubMed ID: 19270692
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