Ubiquitin-binding protein RAP80 mediates BRCA1-dependent DNA damage response.

Mutations in the breast cancer susceptibility gene 1 (BRCA1) are associated with an increased risk of breast and ovarian cancers. BRCA1 participates in the cellular DNA damage response. We report the identification of receptor-associated protein 80 (RAP80) as a BRCA1-interacting protein in humans. RAP80 contains a tandem ubiquitin-interacting motif domain, ...
which is required for its binding with ubiquitin in vitro and its damage-induced foci formation in vivo. Moreover, RAP80 specifically recruits BRCA1 to DNA damage sites and functions with BRCA1 in G2/M checkpoint control. Together, these results suggest the existence of a ubiquitination-dependent signaling pathway involved in the DNA damage response.
Mesh Terms:
Amino Acid Sequence, BRCA1 Protein, Carrier Proteins, Cell Cycle, Cell Line, Tumor, DNA, DNA Damage, DNA Repair, Hela Cells, Humans, Molecular Sequence Data, Nuclear Proteins, Protein Binding, Protein Structure, Tertiary, RNA, Small Interfering, Radiation, Ionizing, Ubiquitin
Science
Date: May. 25, 2007
Download Curated Data For This Publication
96876
Switch View:
  • Interactions 4