Sdo1p, the yeast orthologue of Shwachman-Bodian-Diamond syndrome protein, binds RNA and interacts with nuclear rRNA-processing factors.
The Shwachman-Bodian-Diamond syndrome protein (SBDS) is a member of a highly conserved protein family of not well understood function, with putative orthologues found in different organisms ranging from Archaea, yeast and plants to vertebrate animals. The yeast orthologue of SBDS, Sdo1p, has been previously identified in association with the 60S ... ribosomal subunit and is proposed to participate in ribosomal recycling. Here we show that Sdo1p interacts with nucleolar rRNA processing factors and ribosomal proteins, indicating that it might bind the pre-60S complex and remain associated with it during processing and transport to the cytoplasm. Corroborating the protein interaction data, Sdo1p localizes to the nucleus and cytoplasm and co-immunoprecipitates precursors of 60S and 40S subunits, as well as the mature rRNAs. Sdo1p binds RNA directly, suggesting that it may associate with the ribosomal subunits also through RNA interaction.
Mesh Terms:
Amino Acid Motifs, Biological Transport, Cell Nucleus, Cytoplasm, Humans, Models, Biological, Molecular Weight, Proteins, RNA Processing, Post-Transcriptional, RNA, Fungal, RNA, Ribosomal, RNA-Binding Proteins, Recombinant Proteins, Ribosomal Proteins, Ribosome Subunits, Large, Eukaryotic, Ribosome Subunits, Small, Eukaryotic, Saccharomyces cerevisiae Proteins
Amino Acid Motifs, Biological Transport, Cell Nucleus, Cytoplasm, Humans, Models, Biological, Molecular Weight, Proteins, RNA Processing, Post-Transcriptional, RNA, Fungal, RNA, Ribosomal, RNA-Binding Proteins, Recombinant Proteins, Ribosomal Proteins, Ribosome Subunits, Large, Eukaryotic, Ribosome Subunits, Small, Eukaryotic, Saccharomyces cerevisiae Proteins
Yeast
Date: May. 01, 2009
PubMed ID: 19350533
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