Yvh1 is required for a late maturation step in the 60S biogenesis pathway.

Before entering translation, preribosomal particles undergo sequential late maturation steps. In the case of pre-60S particles, these steps involve the release of shuttling maturation factors and transport receptors. In this study, we report a new maturation step in the 60S biogenesis pathway in budding yeast. We show that efficient release ...
of the nucleolar/nuclear ribosomal-like protein Mrt4 (homologous to the acidic ribosomal P-protein Rpp0) from pre-60S particles requires the highly conserved protein Yvh1, which associates only with late pre-60S particles. Cell biological and biochemical analyses reveal that Mrt4 fails to dissociate from late pre-60S particles in yvh1Delta cells, inducing a delay in nuclear pre-ribosomal RNA processing and a pre-60S export defect in yvh1Delta cells. Moreover, we have isolated gain of function alleles of Mrt4 that specifically bypass the requirement for Yvh1 and rescue all yvh1Delta-associated phenotypes. Together, our data suggest that Yvh1-mediated release of Mrt4 precedes cytoplasmic loading of Rpp0 on pre-60S particles and is an obligatory late step toward construction of translation-competent 60S subunits.
Mesh Terms:
Active Transport, Cell Nucleus, Amino Acid Sequence, Binding Sites, Dual-Specificity Phosphatases, Gene Expression Regulation, Fungal, Genotype, Models, Molecular, Molecular Sequence Data, Mutation, Phenotype, Protein Conformation, Ribosomal Proteins, Ribosome Subunits, Large, Eukaryotic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time Factors
J. Cell Biol.
Date: Sep. 21, 2009
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