Role of Saccharomyces cerevisiae oxidoreductases Bdh1p and Ara1p in the metabolism of acetoin and 2,3-butanediol.

NAD-dependent butanediol dehydrogenase (Bdh1p) from Saccharomyces cerevisiae reversibly transforms acetoin to 2,3-butanediol in a stereospecific manner. Deletion of BDH1 resulted in an accumulation of acetoin and a diminution of 2,3-butanediol in two S. cerevisiae strains under two different growth conditions. The concentrations of (2R,3R)-2,3-butanediol are mostly dependent on Bdh1p activity, ...
while those of (meso)-2,3-butanediol are also influenced by the activity of NADP(H)-dependent oxidoreductases. One of them has been purified and shown to be d-arabinose dehydrogenase (Ara1p), which converts (R/S)-acetoin to meso-2,3-butanediol and (2S,3S)-2,3-butanediol. Deletion of BDH2, a gene adjacent to BDH1, whose encoded protein is 51% identical to Bdh1p, does not significantly alter the levels of acetoin or 2,3-butanediol in comparison to the wild-type strain. Furthermore, we have expressed Bdh2p with a histidine tag and have shown it to be inactive toward 2,3-butanediol. A whole-genome expression analysis with microarrays demonstrates that BDH1 and BDH2 are reciprocally regulated.
Mesh Terms:
Acetoin, Aerobiosis, Alcohol Oxidoreductases, Amino Acid Substitution, Anaerobiosis, Butylene Glycols, Cloning, Molecular, Conserved Sequence, Fermentation, Gene Deletion, Genetic Engineering, Hydrogen-Ion Concentration, Kinetics, Mutation, NAD, Oxidoreductases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Substrate Specificity, Sugar Alcohol Dehydrogenases
Appl. Environ. Microbiol.
Date: Feb. 01, 2010
Download Curated Data For This Publication
97364
Switch View:
  • Interactions 1