Genome-wide localization analysis of a complete set of Tafs reveals a specific effect of the taf1 mutation on Taf2 occupancy and provides indirect evidence for different TFIID conformations at different promoters.
In Saccharomyces cerevisiae, TFIID and SAGA principally mediate transcription of constitutive housekeeping genes and stress-inducible genes, respectively, by delivering TBP to the core promoter. Both are multi-protein complexes composed of 15 and 20 subunits, respectively, five of which are common and which may constitute a core sub-module in each complex. ... Although genome-wide gene expression studies have been conducted extensively in several TFIID and/or SAGA mutants, there are only a limited number of studies investigating genome-wide localization of the components of these two complexes. Specifically, there are no previous reports on localization of a complete set of Tafs and the effects of taf mutations on localization. Here, we examine the localization profiles of a complete set of Tafs, Gcn5, Bur6/Ncb2, Sua7, Tfa2, Tfg1, Tfb3 and Rpb1, on chromosomes III, IV and V by chromatin immunoprecipitation (ChIP)-chip analysis in wild-type and taf1-T657K mutant strains. In addition, we conducted conventional and sequential ChIP analysis of several ribosomal protein genes (RPGs) and non-RPGs. Intriguingly, the results revealed a novel relationship between TFIIB and NC2, simultaneous co-localization of SAGA and TFIID on RPG promoters, specific effects of taf1 mutation on Taf2 occupancy, and an indirect evidence for the existence of different TFIID conformations.
Mesh Terms:
Gene Expression Regulation, Fungal, Genome, Fungal, Mutation, Promoter Regions, Genetic, Protein Conformation, Repressor Proteins, Ribosomal Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, TATA-Binding Protein Associated Factors, Trans-Activators, Transcription Factor TFIIB, Transcription Factor TFIID
Gene Expression Regulation, Fungal, Genome, Fungal, Mutation, Promoter Regions, Genetic, Protein Conformation, Repressor Proteins, Ribosomal Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, TATA-Binding Protein Associated Factors, Trans-Activators, Transcription Factor TFIIB, Transcription Factor TFIID
Nucleic Acids Res.
Date: Apr. 01, 2010
PubMed ID: 20026583
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