CHD1 associates with NCoR and histone deacetylase as well as with RNA splicing proteins.

CHD1 is one of a family of nuclear proteins containing two chromodomains, a SWI/SNF-like helicase/ATPase domain and a DNA binding domain. We found that CHD1 co-immunoprecipitates with histone deacetylase (HDAC) activity and that CHD1 also associates with NCoR, a transcriptional corepressor, in yeast two-hybrid and in vitro pull-down assays. NCoR ...
is known to associate with HDACs to effect its repressive activity, suggesting that the predicted chromatin remodeling activity of CHD1 plays a role in this repression. Yeast two-hybrid assays also showed that CHD1 interacts with splicing proteins mKIAA0164, Srp20, and SAF-B. Splicing assays show that CHD1 overexpression can affect alternative splicing. These results suggest that CHD1 may function in both chromatin mediated transcriptional repression and RNA splicing.
Mesh Terms:
Base Sequence, Cell Line, DNA Helicases, DNA Primers, DNA-Binding Proteins, Histone Deacetylases, Humans, Nuclear Proteins, Nuclear Receptor Co-Repressor 1, Protein Binding, RNA Splicing, Repressor Proteins
Biochem. Biophys. Res. Commun.
Date: Aug. 15, 2003
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