A coactivator of pre-mRNA splicing.

The nuclear matrix antigen recognized by the monoclonal antibody (mAb) B1C8 is a novel serine (S) and arginine (R)-rich protein associated with splicing complexes and is named here SRm160 (SR-related matrix protein of 160 kD). SRm160 contains multiple SR repeats, but unlike proteins of the SR family of splicing factors, ...
lacks an RNA recognition motif. SRm160 and a related protein SRm300 (the 300-kD nuclear matrix antigen recognized by mAb B4A11) form a complex that is required for the splicing of specific pre-mRNAs. The SRm160/300 complex associates with splicing complexes and promotes splicing through interactions with SR family proteins. Binding of SRm160/300 to pre-mRNA is normally also dependent on U1 snRNP and is stabilized by U2 snRNP. Thus, SRm160/300 forms multiple interactions with components bound directly to important sites within pre-mRNA. The results suggest that a complex of the nuclear matrix proteins SRm160 and SRm300 functions as a coactivator of pre-mRNA splicing.
Mesh Terms:
Amino Acid Sequence, Antigens, Nuclear, Bacterial Proteins, Hela Cells, Humans, Interphase, Lymphoma, Large B-Cell, Diffuse, Metaphase, Molecular Sequence Data, Nuclear Matrix, Nuclear Matrix-Associated Proteins, Nuclear Proteins, RNA Precursors, RNA-Binding Proteins, Ribonucleoprotein, U1 Small Nuclear, Ribonucleoprotein, U2 Small Nuclear, Sequence Alignment, Sequence Homology, Amino Acid, Spliceosomes, Tumor Cells, Cultured
Genes Dev.
Date: Apr. 01, 1998
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