Crystal structure of the 14-3-3zeta:serotonin N-acetyltransferase complex. a role for scaffolding in enzyme regulation.
Serotonin N-acetyltransferase (AANAT) controls the daily rhythm in melatonin synthesis. When isolated from tissue, AANAT copurifies with isoforms epsilon and zeta of 14-3-3. We have determined the structure of AANAT bound to 14-3-3zeta, an association that is phosphorylation dependent. AANAT is bound in the central channel of the 14-3-3zeta dimer, ... and is held in place by extensive interactions both with the amphipathic phosphopeptide binding groove of 14-3-3zeta and with other parts of the central channel. Thermodynamic and activity measurements, together with crystallographic analysis, indicate that binding of AANAT by 14-3-3zeta modulates AANAT's activity and affinity for its substrates by stabilizing a region of AANAT involved in substrate binding.
Mesh Terms:
14-3-3 Proteins, Animals, Arylamine N-Acetyltransferase, Calorimetry, Crystallography, X-Ray, Genes, Reporter, Humans, Models, Molecular, Protein Binding, Protein Structure, Quaternary, Protein Structure, Tertiary, Recombinant Fusion Proteins, Sheep, Tyrosine 3-Monooxygenase
14-3-3 Proteins, Animals, Arylamine N-Acetyltransferase, Calorimetry, Crystallography, X-Ray, Genes, Reporter, Humans, Models, Molecular, Protein Binding, Protein Structure, Quaternary, Protein Structure, Tertiary, Recombinant Fusion Proteins, Sheep, Tyrosine 3-Monooxygenase
Cell
Date: Apr. 20, 2001
PubMed ID: 11336675
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