TIN2, a new regulator of telomere length in human cells.
Telomeres are DNA-protein structures that cap linear chromosomes and are essential for maintaining genomic stability and cell phenotype. We identified a novel human telomere-associated protein, TIN2, by interaction cloning using the telomeric DNA-binding-protein TRF1 as a bait. TIN2 interacted with TRF1 in vitro and in cells, and co-localized with TRF1 ... in nuclei and metaphase chromosomes. A mutant TIN2 that lacks amino-terminal sequences effects elongated human telomeres in a telomerase-dependent manner. Our findings suggest that TRF1 is insufficient for control of telomere length in human cells, and that TIN2 is an essential mediator of TRF1 function.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Cell Line, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Gene Expression, Humans, Molecular Sequence Data, RNA, Messenger, Recombinant Proteins, Sequence Deletion, Telomere, Telomere-Binding Proteins, Telomeric Repeat Binding Protein 1, Tissue Distribution
Amino Acid Sequence, Base Sequence, Cell Line, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Gene Expression, Humans, Molecular Sequence Data, RNA, Messenger, Recombinant Proteins, Sequence Deletion, Telomere, Telomere-Binding Proteins, Telomeric Repeat Binding Protein 1, Tissue Distribution
Nat. Genet.
Date: Dec. 01, 1999
PubMed ID: 10581025
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