Molecular mechanisms of coupled monoubiquitination.
Many proteins contain ubiquitin-binding domains or motifs (UBDs), such as the UIM (ubiquitin-interacting motif) and are referred to as ubiquitin receptors. Ubiquitin receptors themselves are frequently monoubiquitinated by a process that requires the presence of a UBD and is referred to as coupled monoubiquitination. Using a UIM-containing protein, eps15, as ... a model, we show here that coupled monoubiquitination strictly depends on the ability of the UIM to bind to monoubiquitin (mUb). We found that the underlying molecular mechanism is based on interaction between the UIM and a ubiquitin ligase (E3), which has itself been modified by ubiquitination. Furthermore, we demonstrate that the in vivo ubiquitination of members of the Nedd4 family of E3 ligases correlates with their ability to monoubiquitinate eps15. Thus, our results clarify the mechanism of coupled monoubiquitination and identify the ubiquitination of E3 ligases as a critical determinant in this process.
Mesh Terms:
Binding Sites, Calcium-Binding Proteins, Catalysis, Endosomal Sorting Complexes Required for Transport, Hela Cells, Humans, Immunoblotting, Intracellular Signaling Peptides and Proteins, Models, Biological, Mutation, Phosphoproteins, Protein Binding, Transfection, Ubiquitin, Ubiquitin-Protein Ligases
Binding Sites, Calcium-Binding Proteins, Catalysis, Endosomal Sorting Complexes Required for Transport, Hela Cells, Humans, Immunoblotting, Intracellular Signaling Peptides and Proteins, Models, Biological, Mutation, Phosphoproteins, Protein Binding, Transfection, Ubiquitin, Ubiquitin-Protein Ligases
Nat. Cell Biol.
Date: Nov. 01, 2006
PubMed ID: 17013377
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