Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity.
Oct-1 is a transcription factor involved in the cell cycle regulation of histone H2B gene transcription and in the transcription of other cellular housekeeping genes. Oct-1 is hyperphosphorylated as cells enter mitosis, and mitosis-specific phosphorylation is reversed as cells exit mitosis. A mitosis-specific phosphorylation site in the homeodomain of Oct-1 ... was phosphorylated in vitro by protein kinase A. Phosphorylation of this site correlated with inhibition of Oct-1 DNA binding activity in vivo and in vitro. The inhibition of Oct-1 DNA binding during mitosis suggests a mechanism by which the general inhibition of transcription during mitosis might occur.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Cattle, Cell Cycle, Cloning, Molecular, DNA, Neoplasm, DNA-Binding Proteins, Genes, Homeobox, Hela Cells, Histones, Host Cell Factor C1, Humans, Mitosis, Molecular Sequence Data, Myocardium, Octamer Transcription Factor-1, Oligodeoxyribonucleotides, Peptide Mapping, Phosphopeptides, Phosphorylation, Protein Kinases, Transcription Factors
Amino Acid Sequence, Animals, Base Sequence, Cattle, Cell Cycle, Cloning, Molecular, DNA, Neoplasm, DNA-Binding Proteins, Genes, Homeobox, Hela Cells, Histones, Host Cell Factor C1, Humans, Mitosis, Molecular Sequence Data, Myocardium, Octamer Transcription Factor-1, Oligodeoxyribonucleotides, Peptide Mapping, Phosphopeptides, Phosphorylation, Protein Kinases, Transcription Factors
Science
Date: Dec. 20, 1991
PubMed ID: 1684878
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