BAIT

RAD8

SPAC5H10.14c, SPAC13G6.01c
ubiquitin-protein ligase E3 Rad8
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)
PREY

RAD55

rhp55, SPAC3C7.03c
RecA family ATPase Rad55/Rhp55
Schizosaccharomyces pombe (972h)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Essential Domains of Schizosaccharomyces pombe Rad8 Required for DNA Damage Response.

Ding L, Forsburg SL

Schizosaccharomyces pombe Rad8 is a conserved protein homologous to Saccharomyces cerevisiae Rad5 and human HLTF that is required for error-free post-replication repair by contributing to polyubiquitylation of PCNA. It has three conserved domains: an E3 ubiquitin ligase motif, a SNF2-family helicase domain, and a family-specific HIRAN domain. Data from humans and budding yeast suggest that helicase activity contributes to replication ... [more]

G3 (Bethesda) May. 28, 2014; 0(0); [Pubmed: 24875629]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: resistance to chemicals (APO:0000087)
  • phenotype: uv resistance (APO:0000085)

Additional Notes

  • Table S4
  • sensitivity to HU, MMS, UV, CPT

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAD55 RAD8
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-10.0317BioGRID
774243
RAD8 RAD55
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-10.0317BioGRID
755307
RAD8 RAD55
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

High-BioGRID
338774

Curated By

  • BioGRID