BAIT

RAD8

SPAC5H10.14c, SPAC13G6.01c
ubiquitin-protein ligase E3 Rad8
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)
PREY

RAD57

rhp57, SPAC145.01, SPAC20H4.07
RecA family ATPase Rad57/Rhp57
GO Process (4)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Essential Domains of Schizosaccharomyces pombe Rad8 Required for DNA Damage Response.

Ding L, Forsburg SL

Schizosaccharomyces pombe Rad8 is a conserved protein homologous to Saccharomyces cerevisiae Rad5 and human HLTF that is required for error-free post-replication repair by contributing to polyubiquitylation of PCNA. It has three conserved domains: an E3 ubiquitin ligase motif, a SNF2-family helicase domain, and a family-specific HIRAN domain. Data from humans and budding yeast suggest that helicase activity contributes to replication ... [more]

G3 (Bethesda) May. 28, 2014; 0(0); [Pubmed: 24875629]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: resistance to chemicals (APO:0000087)
  • phenotype: uv resistance (APO:0000085)

Additional Notes

  • Table S4
  • sensitivity to HU, MMS, UV, CPT

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAD8 RAD57
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-12.3312BioGRID
522543
RAD8 RAD57
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-8.4074BioGRID
755316
RAD57 RAD8
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-8.4074BioGRID
755436

Curated By

  • BioGRID