BAIT

SEC61

translocon subunit SEC61, L000001852, YLR378C
Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex allows SRP-independent protein import into ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

PRE1

proteasome core particle subunit beta 4, L000001483, YER012W
Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
UPS Project
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Degradation of subunits of the Sec61p complex, an integral component of the ER membrane, by the ubiquitin-proteasome pathway.

Biederer T, Volkwein C, Sommer T

We have investigated the degradation of subunits of the trimeric Sec61p complex, a key component of the protein translocation apparatus of the ER membrane. A mutant form of Sec6lp and one of the two associated proteins (Sss1p) are selectively degraded, while the third constituent of the complex (Sbh1p) is stable. Our results demonstrate that the proteolysis of the multispanning membrane ... [more]

EMBO J. May. 01, 1996; 15(9);2069-76 [Pubmed: 8641272]

Throughput

  • Low Throughput

Ontology Terms

  • protein/peptide accumulation (APO:0000149)

Additional Notes

  • sec61-2 and pre1-1 alleles; mutant Sec61-2p is stabilized at elevated temperature by pre1-1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PRE1 SEC61
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1788BioGRID
1929380

Curated By

  • BioGRID