BAIT

LDB2

CLIM1, LDB1
LIM domain binding 2
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

RILP

PP10141
Rab interacting lysosomal protein
GO Process (3)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Construction and characterization of a normalized yeast two-hybrid library derived from a human protein-coding clone collection.

DeGrado-Warren J, Dufford M, Chen J, Bartel PL, Shattuck D, Frech GC

The nuclear yeast two-hybrid (Y2H) system is the most widely used technology for detecting interactions between proteins. A common approach is to screen specific test proteins (baits) against large compilations of randomly cloned proteins (prey libraries). For eukaryotic organisms, libraries have traditionally been generated using messenger RNA (mRNA) extracted from various tissues and cells. Here we present a library construction ... [more]

BioTechniques Feb. 01, 2008; 44(2);265-73 [Pubmed: 18330356]

Throughput

  • Low Throughput

Curated By

  • BioGRID