BAIT

BDF2

SPAC631.02, nrc1, SPAC631.02
BET family double bromodomain protein Bdf2
GO Process (2)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)
PREY

MMS19

SPAC1071.02
Dos2 silencing complex subunit Mms19
GO Process (2)
GO Function (0)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J, Tadeo X, Hou H, Tu PG, Thompson J, Yates JR, Jia S

Heterochromatin spreading leads to the silencing of genes within its path, and boundary elements have evolved to constrain such spreading. In fission yeast, heterochromatin at centromeres I and III is flanked by inverted repeats termed IRCs, which are required for proper boundary functions. However, the mechanisms by which IRCs prevent heterochromatin spreading are unknown. Here, we identified Bdf2, which is ... [more]

Genes Dev. Sep. 01, 2013; 27(17);1886-902 [Pubmed: 24013502]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MMS19 BDF2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-9.0125BioGRID
523033

Curated By

  • BioGRID