BAIT
SAW1
SPBC409.16c, SPBC409.16c
recombination protein Saw1 (predicted)
GO Process (1)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
Schizosaccharomyces pombe (972h)
PREY
DNA2
SPBC16D10.04c
DNA replication endonuclease-helicase Dna2
GO Process (10)
GO Function (7)
GO Component (6)
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [ISO]
- DNA catabolic process, exonucleolytic [IDA]
- DNA duplex unwinding [ISO]
- DNA repair [ISS]
- removal of RNA primer involved in mitotic DNA replication [IMP]
- replication fork processing [IMP]
- replication fork reversal [IMP]
- response to intra-S DNA damage checkpoint signaling [IMP]
- telomere maintenance [IGI]
- telomeric 3' overhang formation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Fission Yeast Pxd1 Promotes Proper DNA Repair by Activating Rad16XPF and Inhibiting Dna2.
Structure-specific nucleases play crucial roles in many DNA repair pathways. They must be precisely controlled to ensure optimal repair outcomes; however, mechanisms of their regulation are not fully understood. Here, we report a fission yeast protein, Pxd1, that binds to and regulates two structure-specific nucleases: Rad16XPF-Swi10ERCC1 and Dna2-Cdc24. Strikingly, Pxd1 influences the activities of these two nucleases in opposite ways: ... [more]
PLoS Biol. Sep. 01, 2014; 12(9);e1001946 [Pubmed: 25203555]
Throughput
- Low Throughput
Additional Notes
- Figure 1
Curated By
- BioGRID