BAIT

RGA7

SPBC23G7.08c
Rho-type GTPase activating protein Rga7
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)
PREY

IMP2

SPBC11C11.02
contractile ring protein Imp2
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

F-BAR domain protein Rga7 collaborates with Cdc15 and Imp2 to ensure proper cytokinesis in fission yeast.

Martin-Garcia R, Coll PM, Perez P

F-BAR domain proteins act as linkers between the cell cortex and cytoskeleton and are involved in membrane binding and bending. Rga7 is one of the seven F-BAR proteins present in fission yeast. In addition to the F-BAR domain at the N-terminal region, Rga7 possesses a Rho-GAP domain at its C-terminus. We show here that Rga7 is necessary to prevent contracting ... [more]

J. Cell. Sci. Jul. 22, 2014; 0(0); [Pubmed: 25052092]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • Figure 6
  • rga7 imp2 double mutant is synthetic lethal

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IMP2 RGA7
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
1026524
IMP2 RGA7
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
1026523

Curated By

  • BioGRID