BAIT

SPT10

CRE1, SUD1, L000002035, YJL127C
Putative histone acetylase with a role in transcriptional silencing; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box
Saccharomyces cerevisiae (S288c)
PREY

TAF9

TAF17, TATA-binding protein-associated factor TAF9, TafII17, L000003459, YMR236W
Subunit (17 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Cell cycle-regulated oscillator coordinates core histone gene transcription through histone acetylation.

Kurat CF, Lambert JP, Petschnigg J, Friesen H, Pawson T, Rosebrock A, Gingras AC, Fillingham J, Andrews B

DNA replication occurs during the synthetic (S) phase of the eukaryotic cell cycle and features a dramatic induction of histone gene expression for concomitant chromatin assembly. Ectopic production of core histones outside of S phase is toxic, underscoring the critical importance of regulatory pathways that ensure proper expression of histone genes. Several regulators of histone gene expression in the budding ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 16, 2014; 0(0); [Pubmed: 25228766]

Throughput

  • High Throughput

Additional Notes

  • Figure S1, Dataset S1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TAF9 SPT10
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low/High-BioGRID
425538

Curated By

  • BioGRID