BAIT

SPT10

CRE1, SUD1, L000002035, YJL127C
Putative histone acetylase with a role in transcriptional silencing; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box
Saccharomyces cerevisiae (S288c)
PREY

SMC1

CHL10, cohesin subunit SMC1, L000001926, YFL008W
Subunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
GO Process (3)
GO Function (4)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Cell cycle-regulated oscillator coordinates core histone gene transcription through histone acetylation.

Kurat CF, Lambert JP, Petschnigg J, Friesen H, Pawson T, Rosebrock A, Gingras AC, Fillingham J, Andrews B

DNA replication occurs during the synthetic (S) phase of the eukaryotic cell cycle and features a dramatic induction of histone gene expression for concomitant chromatin assembly. Ectopic production of core histones outside of S phase is toxic, underscoring the critical importance of regulatory pathways that ensure proper expression of histone genes. Several regulators of histone gene expression in the budding ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 16, 2014; 0(0); [Pubmed: 25228766]

Throughput

  • High Throughput

Additional Notes

  • Figure S1, Dataset S1
  • SPT10 overexpression

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SMC1 SPT10
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
818282

Curated By

  • BioGRID