BAIT

SPT21

L000002040, YMR179W
Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

SRO9

L000003106, YCL037C
Cytoplasmic RNA-binding protein; shuttles between nucleus and cytoplasm and is exported from the nucleus in an mRNA export-dependent manner; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Cell cycle-regulated oscillator coordinates core histone gene transcription through histone acetylation.

Kurat CF, Lambert JP, Petschnigg J, Friesen H, Pawson T, Rosebrock A, Gingras AC, Fillingham J, Andrews B

DNA replication occurs during the synthetic (S) phase of the eukaryotic cell cycle and features a dramatic induction of histone gene expression for concomitant chromatin assembly. Ectopic production of core histones outside of S phase is toxic, underscoring the critical importance of regulatory pathways that ensure proper expression of histone genes. Several regulators of histone gene expression in the budding ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 16, 2014; 0(0); [Pubmed: 25228766]

Throughput

  • High Throughput

Additional Notes

  • Figure S1, Dataset S1
  • SPT21 overexpression

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SPT21 SRO9
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-6.4162BioGRID
216158

Curated By

  • BioGRID