MAPKAP1
Gene Ontology Biological Process
Gene Ontology Molecular Function- Ras GTPase binding [ISO]
- phosphatidic acid binding [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- phosphatidylinositol-3,4-bisphosphate binding [ISO]
- phosphatidylinositol-3,5-bisphosphate binding [ISO]
- phosphatidylinositol-4,5-bisphosphate binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- Ras GTPase binding [ISO]
- phosphatidic acid binding [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- phosphatidylinositol-3,4-bisphosphate binding [ISO]
- phosphatidylinositol-3,5-bisphosphate binding [ISO]
- phosphatidylinositol-4,5-bisphosphate binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
Gene Ontology Cellular Component
RICTOR
Gene Ontology Biological Process
- actin cytoskeleton reorganization [ISO]
- embryo development [IMP]
- peptidyl-serine phosphorylation [ISO]
- positive regulation of TOR signaling [ISO]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of endothelial cell proliferation [ISO]
- positive regulation of peptidyl-tyrosine phosphorylation [IMP]
- positive regulation of protein kinase B signaling [ISO]
- regulation of Rac GTPase activity [IMP]
- regulation of actin cytoskeleton organization [ISO]
- regulation of phosphorylation [ISO]
- regulation of protein kinase B signaling [IDA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- TORC2 complex [IDA, ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Syndecan-4 regulates subcellular localization of mTOR Complex2 and Akt activation in a PKCalpha-dependent manner in endothelial cells.
Mammalian target of rapamycin (mTOR) activity is regulated by assembly of two functionally distinct complexes, mTORC1 and mTORC2. In syndecan-4 (S4) null endothelial cells, mTORC2 activity is reduced, resulting in decreased Akt activation, while mTORC1 activity is increased. Levels of rictor, mLST8, and mSin-1 are unchanged in total cell lysates but decreased in the rafts of S4(-/-) endothelial cells, as ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MAPKAP1 RICTOR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RICTOR MAPKAP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RICTOR MAPKAP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID