BAIT

RPS6KB1

2610318I15Rik, 4732464A07Rik, 70kDa, AA959758, AI256796, AI314060, S6K1, p70/85s6k, p70s6k, RP23-467J12.2
ribosomal protein S6 kinase, polypeptide 1
Mus musculus
PREY

DDIT4L

1700037B15Rik, 1700108M02Rik, REDD2, RTP801L, Smhs1
DNA-damage-inducible transcript 4-like
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Regulation of mTOR and cell growth in response to energy stress by REDD1.

Sofer A, Lei K, Johannessen CM, Ellisen LW

The tuberous sclerosis tumor suppressors TSC1 and TSC2 regulate the mTOR pathway to control translation and cell growth in response to nutrient and growth factor stimuli. We have recently identified the stress response REDD1 gene as a mediator of tuberous sclerosis complex (TSC)-dependent mTOR regulation by hypoxia. Here, we demonstrate that REDD1 inhibits mTOR function to control cell growth in ... [more]

Mol. Cell. Biol. Jul. 01, 2005; 25(14);5834-45 [Pubmed: 15988001]

Throughput

  • Low Throughput

Curated By

  • BioGRID