MID1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PPP2R4
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- mitotic spindle organization in nucleus [IBA]
- negative regulation of phosphoprotein phosphatase activity [IDA]
- negative regulation of protein dephosphorylation [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of phosphoprotein phosphatase activity [IDA]
- positive regulation of protein dephosphorylation [IDA]
- protein peptidyl-prolyl isomerization [IBA]
- regulation of phosphoprotein phosphatase activity [IDA]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATPase activity [IDA]
- peptidyl-prolyl cis-trans isomerase activity [IBA]
- protein heterodimerization activity [TAS]
- protein homodimerization activity [IDA]
- protein phosphatase 2A binding [IDA]
- protein phosphatase type 2A regulator activity [IDA, TAS]
- protein tyrosine phosphatase activator activity [IDA]
- receptor binding [IPI]
- ATP binding [IDA]
- ATPase activity [IDA]
- peptidyl-prolyl cis-trans isomerase activity [IBA]
- protein heterodimerization activity [TAS]
- protein homodimerization activity [IDA]
- protein phosphatase 2A binding [IDA]
- protein phosphatase type 2A regulator activity [IDA, TAS]
- protein tyrosine phosphatase activator activity [IDA]
- receptor binding [IPI]
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
MID1 Catalyzes the Ubiquitination of Protein Phosphatase 2A and Mutations within Its Bbox1 Domain Disrupt Polyubiquitination of Alpha4 but Not of PP2Ac.
MID1 is a microtubule-associated protein that belongs to the TRIM family. MID1 functions as an ubiquitin E3 ligase, and recently was shown to catalyze the polyubiquitination of, alpha4, a protein regulator of protein phosphatase 2A (PP2A). It has been hypothesized that MID1 regulates PP2A, requiring the intermediary interaction with alpha4. Here we report that MID1 catalyzes the in vitro ubiquitination ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PPP2R4 MID1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MID1 PPP2R4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 719089 |
Curated By
- BioGRID