PREY

MUL1

C1orf166, GIDE, MAPL, MULAN, RNF218, RP11-401M16.2
mitochondrial E3 ubiquitin protein ligase 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mulan E3 ubiquitin ligase interacts with multiple E2 conjugating enzymes and participates in mitophagy by recruiting GABARAP.

Ambivero CT, Cilenti L, Main S, Zervos AS

Mulan is an E3 ubiquitin ligase embedded in the outer mitochondrial membrane (OMM) with its RING finger facing the cytoplasm and a large domain located in the intermembrane space (IMS). Mulan is known to have an important role in cell growth, cell death, and more recently in mitophagy. The mechanism of its function is poorly understood; but as an E3 ... [more]

Cell. Signal. Sep. 16, 2014; 26(12);2921-2929 [Pubmed: 25224329]

Throughput

  • Low Throughput

Additional Notes

  • in the presence of Ube2e3

Curated By

  • BioGRID