BAIT

HUWE1

ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, URE-B1, UREB1, RP3-339A18.4
HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
UPS Project
Homo sapiens
PREY

MDH2

M-MDH, MDH, MGC:3559, MOR1
malate dehydrogenase 2, NAD (mitochondrial)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative diGly Proteomics Coupled with Inducible RNAi Reveals Ubiquitin-Mediated Proteolysis of DDIT4 by the E3 Ligase HUWE1.

Thompson JW, Nagel J, Hoving S, Gerrits B, Bauer A, Thomas JR, Kirschner MW, Schirle M, Luchansky SJ

Targeted degradation of proteins through the ubiquitin-proteasome system (UPS) via the activities of E3 ubiquitin ligases regulates diverse cellular processes, and misregulation of these enzymes contributes to the pathogenesis of human diseases. One of the challenges facing the UPS field is to delineate the complete cohort of substrates for a particular E3 ligase. Advances in mass spectrometry and the development ... [more]

J. Biol. Chem. Aug. 21, 2014; 0(0); [Pubmed: 25147182]

Throughput

  • High Throughput

Curated By

  • BioGRID