BAIT
HUWE1
ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, URE-B1, UREB1, RP3-339A18.4
HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
GO Process (5)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
EIF4A3
DDX48, MUK34, NMP265, NUK34, RCPS, eIF4AIII
eukaryotic translation initiation factor 4A3
GO Process (12)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- RNA metabolic process [TAS]
- cytokine-mediated signaling pathway [TAS]
- embryonic cranial skeleton morphogenesis [IMP]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- mRNA splicing, via spliceosome [IC]
- negative regulation of translation [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [IMP, TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of translation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Quantitative diGly Proteomics Coupled with Inducible RNAi Reveals Ubiquitin-Mediated Proteolysis of DDIT4 by the E3 Ligase HUWE1.
Targeted degradation of proteins through the ubiquitin-proteasome system (UPS) via the activities of E3 ubiquitin ligases regulates diverse cellular processes, and misregulation of these enzymes contributes to the pathogenesis of human diseases. One of the challenges facing the UPS field is to delineate the complete cohort of substrates for a particular E3 ligase. Advances in mass spectrometry and the development ... [more]
J. Biol. Chem. Aug. 21, 2014; 0(0); [Pubmed: 25147182]
Throughput
- High Throughput
Curated By
- BioGRID