BAIT
HUWE1
ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, URE-B1, UREB1, RP3-339A18.4
HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
GO Process (5)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CRK
CRKII, p38
v-crk avian sarcoma virus CT10 oncogene homolog
GO Process (11)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- activation of MAPKK activity [TAS]
- blood coagulation [TAS]
- ephrin receptor signaling pathway [IDA]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- platelet activation [TAS]
- regulation of Rho GTPase activity [IDA]
- regulation of actin cytoskeleton organization [IDA]
- regulation of transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Quantitative diGly Proteomics Coupled with Inducible RNAi Reveals Ubiquitin-Mediated Proteolysis of DDIT4 by the E3 Ligase HUWE1.
Targeted degradation of proteins through the ubiquitin-proteasome system (UPS) via the activities of E3 ubiquitin ligases regulates diverse cellular processes, and misregulation of these enzymes contributes to the pathogenesis of human diseases. One of the challenges facing the UPS field is to delineate the complete cohort of substrates for a particular E3 ligase. Advances in mass spectrometry and the development ... [more]
J. Biol. Chem. Aug. 21, 2014; 0(0); [Pubmed: 25147182]
Throughput
- High Throughput
Curated By
- BioGRID