BAIT
APH1A
6530402N02Rik, APH-1, APH-1A, CGI-78, RP4-790G17.3
APH1A gamma secretase subunit
GO Process (10)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
- Notch receptor processing [IMP, TAS]
- Notch signaling pathway [TAS]
- amyloid precursor protein catabolic process [IMP, TAS]
- apoptotic signaling pathway [TAS]
- membrane protein ectodomain proteolysis [IDA]
- membrane protein intracellular domain proteolysis [IMP, TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of catalytic activity [IDA]
- protein processing [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PSEN1
Ad3h, PS-1, PS1, S182
presenilin 1
GO Process (86)
GO Function (7)
GO Component (35)
Gene Ontology Biological Process
- Cajal-Retzius cell differentiation [IMP]
- L-glutamate transport [IMP]
- Notch receptor processing [IMP]
- Notch signaling pathway [IGI, IMP]
- T cell activation involved in immune response [IGI]
- T cell receptor signaling pathway [IGI]
- activation of MAPKK activity [IMP]
- amyloid precursor protein catabolic process [IMP]
- anagen [IGI]
- autophagic vacuole assembly [IMP]
- autophagy [IMP]
- beta-amyloid formation [IMP]
- beta-amyloid metabolic process [IMP]
- blood vessel development [IMP]
- brain development [IMP]
- brain morphogenesis [IGI]
- calcium ion transmembrane transport [ISO]
- calcium ion transport [IBA]
- canonical Wnt signaling pathway [IBA]
- cell fate specification [IGI]
- cellular calcium ion homeostasis [IMP]
- cellular protein metabolic process [IDA, IGI, IMP]
- cellular response to DNA damage stimulus [IMP]
- cerebral cortex cell migration [IMP]
- cerebral cortex development [IMP]
- choline transport [IMP]
- dorsal/ventral neural tube patterning [IGI]
- embryo development [IGI, IMP]
- embryonic limb morphogenesis [IGI]
- endoplasmic reticulum calcium ion homeostasis [IGI, IMP, ISO]
- epithelial cell proliferation [IGI]
- forebrain development [IGI]
- heart development [IMP]
- heart looping [IGI]
- hematopoietic progenitor cell differentiation [IGI]
- learning or memory [IGI]
- locomotion [IGI]
- membrane protein ectodomain proteolysis [IBA, ISO]
- memory [IGI, IMP]
- mitochondrial transport [IMP]
- myeloid dendritic cell differentiation [IMP]
- myeloid leukocyte differentiation [IGI]
- negative regulation of apoptotic process [IDA, ISO]
- negative regulation of apoptotic signaling pathway [IGI]
- negative regulation of axonogenesis [IMP]
- negative regulation of epidermal growth factor-activated receptor activity [IMP]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of protein kinase activity [IMP]
- negative regulation of protein phosphorylation [IGI]
- negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of ubiquitin-protein transferase activity [IMP]
- neurogenesis [IMP]
- neuron apoptotic process [IGI]
- neuron development [IMP]
- neuron differentiation [IMP]
- neuron migration [IMP]
- positive regulation of MAP kinase activity [IMP]
- positive regulation of apoptotic process [IGI]
- positive regulation of catalytic activity [ISO]
- positive regulation of coagulation [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of protein kinase activity [IDA]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of receptor recycling [IMP]
- post-embryonic development [IMP]
- protein glycosylation [IMP]
- protein maturation [IGI]
- protein processing [IMP, ISO]
- protein transport [IGI]
- proteolysis [IGI]
- regulation of epidermal growth factor-activated receptor activity [IGI]
- regulation of phosphorylation [ISO]
- regulation of protein binding [IGI]
- regulation of resting membrane potential [IMP]
- regulation of synaptic plasticity [IGI, IMP]
- regulation of synaptic transmission, glutamatergic [IMP]
- response to oxidative stress [IGI, IMP]
- segmentation [IMP]
- single organismal cell-cell adhesion [ISO]
- skeletal system morphogenesis [IMP]
- skin morphogenesis [IMP]
- smooth endoplasmic reticulum calcium ion homeostasis [IGI]
- somitogenesis [IGI, IMP]
- synaptic vesicle targeting [IMP]
- thymus development [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA, ISO]
- Z disc [IBA]
- apical plasma membrane [IBA]
- axon [IDA]
- cell [IGI]
- cell cortex [IBA]
- cell surface [IBA, ISO]
- centrosome [ISO]
- ciliary rootlet [IDA]
- cytoplasmic vesicle [IDA]
- dendrite [IDA]
- dendritic shaft [IDA]
- endoplasmic reticulum [IDA, IMP, ISO]
- gamma-secretase complex [ISO]
- growth cone [IDA]
- integral component of plasma membrane [IBA, ISO]
- intracellular [IDA, IMP]
- kinetochore [ISO]
- lysosomal membrane [IBA, ISO]
- membrane [IDA]
- membrane raft [IBA, ISO]
- membrane-bounded organelle [IDA]
- mitochondrial inner membrane [IBA, ISO]
- mitochondrion [ISO]
- neuromuscular junction [IBA, ISO]
- neuron projection [ISO]
- neuronal cell body [IDA]
- nuclear membrane [ISO]
- nuclear outer membrane [ISO]
- nucleus [IDA]
- perinuclear region of cytoplasm [IBA]
- plasma membrane [IDA]
- protein complex [ISO]
- rough endoplasmic reticulum [ISO]
- smooth endoplasmic reticulum [IGI, ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
An alternative spliced mouse presenilin-2 mRNA encodes a novel gamma-secretase inhibitor.
The gamma-secretase, composed of presenilin-1 (PS1) or presenilin-2 (PS2), nicastrin (NCT), anterior pharynx-defective phenotype 1 (APH-1), and PEN-2, is critical for the development of Alzheimer's disease (AD). PSs are autoproteolytically cleaved, producing an N-terminal fragment (NTF) and a hydrophilic loop domain-containing C-terminal fragment. However, the role of the loop domain in the gamma-secretase complex assembly remains unknown. Here, we report ... [more]
FEBS Lett. May. 06, 2009; 583(9);1403-8 [Pubmed: 19376115]
Throughput
- Low Throughput
Curated By
- BioGRID