BAIT

FUB1

YCR076C
Proteasome-binding protein; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Saccharomyces cerevisiae (S288c)
PREY

POC4

DMP1, PBA4, YPL144W
Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
UPS Project
GO Process (2)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

N-terminal α7 deletion of the proteasome 20S core particle substitutes for yeast PI31 function.

Yashiroda H, Toda Y, Otsu S, Takagi K, Mizushima T, Murata S

The proteasome core particle (CP) is a conserved protease complex that is formed by the stacking of two outer α-rings and two inner β-rings. The α-ring is a heteroheptameric ring of α1-α7 subunits and acts as a gate that restricts entry of substrate proteins into the catalytic cavity formed by the two abutting β-rings. Proteasome inhibitor 31-kDa (PI31) was originally ... [more]

Mol. Cell. Biol. Oct. 20, 2014; 0(0); [Pubmed: 25332237]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • Figure 4

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FUB1 POC4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2052BioGRID
2087726
POC4 FUB1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.7303BioGRID
2191705

Curated By

  • BioGRID