A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Dss1 Is a 26S Proteasome Ubiquitin Receptor.

Paraskevopoulos K, Kriegenburg F, Tatham MH, Roesner HI, Medina B, Larsen IB, Brandstrup R, Hardwick KG, Hay RT, Kragelund BB, Hartmann-Petersen R, Gordon C

The ubiquitin-proteasome system is the major pathway for protein degradation in eukaryotic cells. Proteins to be degraded are conjugated to ubiquitin chains that act as recognition signals for the 26S proteasome. The proteasome subunits Rpn10 and Rpn13 are known to bind ubiquitin, but genetic and biochemical data suggest the existence of at least one other substrate receptor. Here, we show ... [more]

Mol. Cell Oct. 08, 2014; 0(0); [Pubmed: 25306921]

Throughput

Low Throughput

Ontology Terms

phenotype: viability (APO:0000111)

Additional Notes

DSS1 expression rescued rhp23 rpn10 rpn13a rpn13b quadruple mutant

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPN15 RPN1302	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Schenstrom SM (2018)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RPN15

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

RPN1302