OTUD7B
Gene Ontology Biological Process
- mucosal immune response [IMP]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IMP, ISO]
- negative regulation of interleukin-8 production [ISO]
- negative regulation of protein localization to nucleus [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- protein K11-linked deubiquitination [ISO]
- protein K48-linked deubiquitination [IMP, ISO]
- protein K63-linked deubiquitination [ISO]
- protein deubiquitination [IDA]
- proteolysis [IMP]
Gene Ontology Molecular Function
APC
Gene Ontology Biological Process
- apoptotic process [TAS]
- canonical Wnt signaling pathway [IC, NAS]
- cell adhesion [NAS]
- cell cycle arrest [IDA]
- cell migration [IMP]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cellular response to DNA damage stimulus [IDA]
- mitotic cytokinesis [IMP]
- mitotic spindle assembly checkpoint [IMP]
- negative regulation of canonical Wnt signaling pathway [IGI]
- negative regulation of cell proliferation [IDA]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of microtubule depolymerization [IDA, IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of protein catabolic process [IC, IGI]
- positive regulation of pseudopodium assembly [IMP]
- protein complex assembly [IDA]
- regulation of attachment of spindle microtubules to kinetochore [IMP, NAS]
- regulation of microtubule-based process [IMP]
- tight junction assembly [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains.
A key effector of the canonical Wnt pathway is beta-catenin, which binds to TCF/LEF factors to promote the transcription of Wnt target genes. In the absence of Wnt stimulation, beta-catenin is phosphorylated constitutively, and modified with K48-linked ubiquitin for subsequent proteasomal degradation. Here, we identify Trabid as a new positive regulator of Wnt signaling in mammalian and Drosophila cells. Trabid ... [more]
Throughput
- Low Throughput
Additional Notes
- Supplement S1
Curated By
- BioGRID