BAIT

HIPK2

PRO0593
homeodomain interacting protein kinase 2
GO Process (21)
GO Function (8)
GO Component (4)

Gene Ontology Cellular Component

Homo sapiens
PREY

XAF1

BIRC4BP, HSXIAPAF1, XIAPAF1
XIAP associated factor 1
GO Process (3)
GO Function (0)
GO Component (1)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

XAF1 directs apoptotic switch of p53 signaling through activation of HIPK2 and ZNF313.

Lee MG, Han J, Jeong SI, Her NG, Lee JH, Ha TK, Kang MJ, Ryu BK, Chi SG

X-linked inhibitor of apoptosis (XIAP)-associated factor 1 (XAF1) is a tumor suppressor that is frequently inactivated in many human cancers. However, the molecular mechanism underlying its growth-inhibitory function remains largely unknown. Here, we report that XAF1 forms a positive feedback loop with p53 and acts as a molecular switch in p53-mediated cell-fate decisions favoring apoptosis over cell-cycle arrest. XAF1 binds ... [more]

Proc. Natl. Acad. Sci. U.S.A. Oct. 13, 2014; 0(0); [Pubmed: 25313037]

Throughput

  • Low Throughput

Additional Notes

  • source of XAF1 not clear

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
XAF1 HIPK2
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
1032930

Curated By

  • BioGRID