An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The dynamic interaction of AMBRA1 with the dynein motor complex regulates mammalian autophagy.

Di Bartolomeo S, Corazzari M, Nazio F, Oliverio S, Lisi G, Antonioli M, Pagliarini V, Matteoni S, Fuoco C, Giunta L, D'Amelio M, Nardacci R, Romagnoli A, Piacentini M, Cecconi F, Fimia GM

Autophagy is an evolutionary conserved catabolic process involved in several physiological and pathological processes such as cancer and neurodegeneration. Autophagy initiation signaling requires both the ULK1 kinase and the BECLIN 1-VPS34 core complex to generate autophagosomes, double-membraned vesicles that transfer cellular contents to lysosomes. In this study, we show that the BECLIN 1-VPS34 complex is tethered to the cytoskeleton through ... [more]

J. Cell Biol. Oct. 04, 2010; 191(1);155-68 [Pubmed: 20921139]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

DYNC1I1

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule binding [ISS]microtubule motor activity [ISS]motor activity [TAS]protein binding [IPI]spectrin binding [IDA]

Gene Ontology Cellular Component

BECN1