CDC20
Gene Ontology Biological Process
- activation of anaphase-promoting complex activity involved in meiotic cell cycle [IMP]
- activation of mitotic anaphase-promoting complex activity [IMP]
- mitotic spindle assembly checkpoint [IPI]
- negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation [IMP]
- positive regulation of mitotic metaphase/anaphase transition [IMP]
- positive regulation of protein catabolic process [IMP]
- regulation of meiosis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DBF4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Multiple mechanisms determine the order of APC/C substrate degradation in mitosis.
The ubiquitin protein ligase anaphase-promoting complex or cyclosome (APC/C) controls mitosis by promoting ordered degradation of securin, cyclins, and other proteins. The mechanisms underlying the timing of APC/C substrate degradation are poorly understood. We explored these mechanisms using quantitative fluorescence microscopy of GFP-tagged APC/C(Cdc20) substrates in living budding yeast cells. Degradation of the S cyclin, Clb5, begins early in mitosis, ... [more]
Throughput
- Low Throughput
Additional Notes
- The in vitro reaction contained E1, Ubc4 [E2] and the APC-Cdc20 [E3].
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DBF4 CDC20 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1521 | BioGRID | 1925139 | |
| CDC20 DBF4 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.184 | BioGRID | 1873141 | |
| CDC20 DBF4 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 0.2083 | BioGRID | 1873140 |
Curated By
- BioGRID