BAIT

APC11

anaphase promoting complex subunit 11, L000004322, YDL008W

Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity

UPS Project

GO Process (2)

GO Function (1)

GO Component (1)

Gene Ontology Biological Process

anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [IMP]

protein ubiquitination [IMP]

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

anaphase-promoting complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

UBC4

E2 ubiquitin-conjugating protein UBC4, L000002407, YBR082C

Ubiquitin-conjugating enzyme (E2); key E2 partner with Ubc1p for the anaphase-promoting complex (APC); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication

UPS Project

GO Process (5)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

cellular response to heat [IDA]

protein monoubiquitination [IDA]

protein polyubiquitination [IDA]

protein ubiquitination [IDA]

ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]

Gene Ontology Molecular Function

protein binding, bridging [IDA]
ubiquitin binding [IDA, IMP]
ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

proteasome complex [IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation.

Passmore LA, Booth CR, Venien-Bryan C, Ludtke SJ, Fioretto C, Johnson LN, Chiu W, Barford D

The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase composed of approximately 13 distinct subunits required for progression through meiosis, mitosis, and the G1 phase of the cell cycle. Despite its central role in these processes, information concerning its composition and structure is limited. Here, we determined the structure of yeast APC/C by cryo-electron microscopy (cryo-EM). Docking of tetratricopeptide repeat ... [more]

Mol. Cell Dec. 22, 2005; 20(6);855-66 [Pubmed: 16364911]

Throughput

Low Throughput

Additional Notes

E2: Ubc4

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBC4 APC11	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2403	BioGRID	2028536
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Burton JL (2011)	Low	-	BioGRID	1060354
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Enquist-Newman M (2008)	Low	-	BioGRID	-
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Foe IT (2011)	Low	-	BioGRID	-
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Foster SA (2012)	Low	-	BioGRID	-
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Girard JR (2015)	Low	-	BioGRID	1515586
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hanna J (2006)	Low	-	BioGRID	-
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	He J (2013)	Low	-	BioGRID	1034549
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Leverson JD (2000)	Low	-	BioGRID	1029389
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Lianga N (2013)	Low	-	BioGRID	1036085
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Lu D (2015)	Low	-	BioGRID	1511977
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Mallory MJ (2007)	Low	-	BioGRID	3323873
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Matsusaka T (2014)	Low	-	BioGRID	1034553
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Oelschlaegel T (2005)	Low	-	BioGRID	1035149
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Ostapenko D (2011)	Low	-	BioGRID	-
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Ostapenko D (2012)	Low	-	BioGRID	1034557
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Ostapenko D (2015)	Low	-	BioGRID	1255298
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Passmore LA (2003)	High	-	BioGRID	1034206
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Rodrigo-Brenni MC (2007)	Low	-	BioGRID	1034552
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Schuyler SC (2009)	Low	-	BioGRID	1034203
APC11 UBC4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zhang Z (2012)	Low	-	BioGRID	735860

Curated By

BioGRID

Interaction Summary

APC11

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

UBC4

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding, bridging [IDA]ubiquitin binding [IDA, IMP]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

Reconstituted Complex

Publication

Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation.

Throughput

Additional Notes

Related interactions

Curated By

protein binding, bridging [IDA]
ubiquitin binding [IDA, IMP]
ubiquitin-protein transferase activity [IDA]