An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

FBXO22 is Required for Optimal Synthesis of the NMDA Receptor Co-agonist D-Serine.

Dikopoltsev E, Foltyn VN, Zehl M, Jensen ON, Mori H, Radzishevsky I, Wolosker H

D-Serine is a physiological activator of NMDA receptors (NMDARs) in the nervous system that mediates several NMDAR-mediated processes, ranging from normal neurotransmission to neurodegeneration. D-Serine is synthesized from L-serine by serine racemase (SR), a brain-enriched enzyme. Yet, little is known about the regulation of D-serine synthesis. We now demonstrate that the F-box only protein 22 (FBXO22) interacts with SR and ... [more]

J. Biol. Chem. Oct. 21, 2014; 0(0); [Pubmed: 25336657]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SRR FBXO22	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Dikopoltsev E (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SRR

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

FBXO22