Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The Ran binding protein RanBPM interacts with Axl and Sky receptor tyrosine kinases.

Hafizi S, Gustafsson A, Stenhoff J, Dahlbaeck B

Axl belongs to a particular subfamily of transmembrane receptor tyrosine kinases, the biological ligand for which is the growth/survival factor Gas6. However, little is known about the molecular mechanisms for Axl activation and signal transduction. We have previously identified a novel interaction between the intracellular domain of Axl and Ran binding protein in microtubule organising centre (RanBPM). In the present ... [more]

Int. J. Biochem. Cell Biol. Nov. 01, 2005; 37(11);2344-56 [Pubmed: 15964779]

Throughput

  • Low Throughput

Curated By

  • BioGRID