BAIT

RAF1

CMD1NN, CRAF, NS5, Raf-1, c-Raf
Raf-1 proto-oncogene, serine/threonine kinase
GO Process (30)
GO Function (5)
GO Component (5)
Homo sapiens
PREY

MYBPC1

LCCS4, MYBPCC, MYBPCS
myosin binding protein C, slow type
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Ran binding protein 9 interacts with Raf kinase but does not contribute to downstream ERK1/2 activation in skeletal myoblasts.

Johnson SE, Winner DG, Wang X

Raf kinase is the upstream activator of MEK1/2 leading to phosphorylation and activation of ERK1/2. Sustained activation of Raf represses skeletal muscle-specific reporter gene transcription and formation of multinucleated myofibers. Inhibition of myogenesis by activated Raf involves downstream ERK1/2 as well as undefined mediators. To identify Raf-interacting proteins that may influence repression of muscle formation, a yeast two-hybrid screen was ... [more]

Biochem. Biophys. Res. Commun. Feb. 10, 2006; 340(2);409-16 [Pubmed: 16364241]

Throughput

  • Low Throughput

Curated By

  • BioGRID