An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

An adaptor protein complex of FRS2Î² and CIN85/CD2AP provides a novel mechanism for ErbB2/HER2 protein downregulation.

Minegishi Y, Shibagaki Y, Mizutani A, Fujita K, Tezuka T, Kinoshita M, Kuroda M, Hattori S, Gotoh N

Overexpression of the ErbB2/HER2 receptor tyrosine kinase contributes to tumorigenesis. However, mechanisms regulating ErbB2 protein levels remain largely unclear. Here, we identified novel mechanisms of ErbB2 downregulation. ErbB2 constitutively binds to an adaptor protein FRS2Î². We found that FRS2Î² bound to CD2AP and CIN85, which induces endosomal trafficking that targets lysosomes. FRS2Î² colocalized with CIN85 in the cytoplasm. Expression of ... [more]

Cancer Sci. Dec. 20, 2012; 0(0); [Pubmed: 23279575]

Throughput

Low Throughput

Additional Notes

Figure 1

Curated By

BioGRID

Interaction Summary

FRS3

Gene Ontology Biological Process

Gene Ontology Molecular Function

fibroblast growth factor receptor binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

SH3KBP1